FRET Peptides(熒光能量共振轉(zhuǎn)移)
FRET肽是研究肽酶特異性的便利工具,由于其反應(yīng)過(guò)程可被連續(xù)監(jiān)測(cè),為酶活性的檢測(cè)提供了一個(gè)便捷的方法。當(dāng)FRET肽是完整的,表現(xiàn)出的是內(nèi)部的熒光猝滅,但當(dāng)供體/受體對(duì)的任何肽鍵斷裂就會(huì)釋放出熒光,此熒光可被連續(xù)檢測(cè),從而可對(duì)酶的活性進(jìn)行定量分析。

1. Fluorescein and Dabcyl:FAM/Lys(Dabcyl)
2. Fluorescein and Tamra: FAM/TAMRA
3. Methoxy-coumarin-acetic-acid(MCA)and 2,4-Dinitrophenyl(DNP): MCA/Lys(Dnp).
4. Ortho-aminobenzoic acid (Abz) and 2,4-dinitrophenyl (Dnp) or N-(2,4-dinitrophenyl)ethylenediamine(EDDnp): Abz/Tyr (NO2), Abz/EDDnp
5. Dabcyl and Glu(EDANS)
6. Cy3 and Lys(BHQ2)



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